Circadian dynamics of locomotor activity and deltamethrin susceptibility in the pine weevil,Hylobius abietis

Rhythmic locomotor activity and daily susceptibility to deltamethrin were tested in the pine weevil,Hylobius abietis (L.) (Coleoptera, Curculionidae), a pest of young conifer plants. In constant darkness, beetles revealed a free-running circadian pattern of locomotor activity (average period 22h : 20min). Under long photoperiod, L:D 18:6, entrainment of motor activity was observed. In the entrained population of the weevils, a peak of locomotor activity occurred at about the beginning of the dark phase, and the minimum occurred in the middle of the light phase. Fluctuations of susceptibility to a standard dose of deltamethrin (0.5 μg/g body weight) varied across the day, and were inversely correlated to the changes in locomotor activity. The importance of considering the daily organization of insect biology in studying insect resistance to pesticides is re-emphasized. 3 www.csa.com. The following databases were screened: Animal Behaviour Abstracts, AGRICOLA, Aquatic Science and Fishery Abstracts, Biological Sciences, Ecology Abstracts, Entomology Abstracts, Environmental Science and Pollution Abstracts, Health and Safety Science Abstracts, Pollution Abstracts, Water Resource Abstracts, Toxicology Abstracts, TOXLINE.     

Immunolocalisation of oestrogen receptors alpha (ERalpha) and beta (ERbeta) in porcine embryos and fetuses at different stages of gestation

The sites of oestrogen action can be shown by the localisation of their receptors in the target tissues. The aim of the present study was to show the localisation of oestrogen receptors in porcine embryos and fetuses obtained on days 18, 22, 32, 40, 50, 60, 71 and 90 post coitum (p.c.). The visualisation of proteins was conducted in embryos and various fetal organs such as gonads, uterus, lung, kidney, intestine and adrenal gland. Both ERs were observed in the blastocysts on day 18 p.c. In the male, ERbeta was detected in the testis and epididymis, whereas ERalpha was present in the efferent ductules. In the female, ERbeta was detected in the ovarian stromal cells investing the oocyte nests, while ERalpha protein was detected in the surface epithelium. In the uterus, ERs were present in the stromal cells, while ERbeta was present in the luminal epithelium. In the non-reproductive fetal porcine tissues ERbeta was localised in the lungs, kidneys, adrenal glands and in the umbilical cords. Both ERs were observed in the intestine. It is possible that ERbeta may play important roles in the development of the adrenal gland, testis, kidney and lungs, while both ERs are involved in the development of the ovary, uterus, epididymis and intestine of the porcine fetus.     

Apoptosis inhibition by insulin-like growth factor (IGF)-I during in vitro maturation of bovine oocytes

Recently, significant progress has been achieved in improving the yield of good quality embryos in vitro. However, efforts are still required to recognize the factors and understand the mechanisms of oocyte maturation, which are essential for subsequent embryo development. The aims of the present study were to determine the frequency of apoptosis in oocytes recovered from slaughterhouse ovaries and to investigate whether insulin-like growth factor (IGF)-I action during oocyte maturation in vitro may withhold apoptosis and improve oocyte quality. Only oocytes of proper morphology with homogenous ooplasm and compact cumulus cells were selected for this study. All oocytes recovered from the slaughterhouse ovaries were divided into two groups. One group of oocytes, chosen for apoptosis detection, was examined immediately after recovery. The other group of oocytes was maturated in vitro. Oocytes were maturated with IGF-I supplementation (100 ng/ml). Oocytes without supplementation were used as a control. Apoptosis in oocytes was determined by positive results of TUNEL assay and active caspase labeling. The percentage of apoptotic oocytes detected by TUNEL fell to zero when the maturation medium was supplemented with IGF-I in comparison to the control matured oocytes (0 vs. 9.87%; P<0.05). However, active caspase labeling was only slightly decreased in the IGF-I matured oocytes compared with the control matured oocytes (1.13 vs. 2.08%; P<0.05). The results indicate that IGF-I may serve as an anti-apoptotic factor during oocyte maturation. We suggest that IGF-I may inhibit apoptosis in oocytes at the stage of caspase activation and may prevent further advancement of oocyte apoptosis.     

Species mixing effect on Norway spruce response to elevated CO2 and climatic variables: root and radial growth response

A 7-year study was conducted to examine the growth (diameter and root) response of Norway spruce (Picea abies (L.) Karst.) seedlings to elevated CO₂ (CO_2ELV, 770 μmol (CO₂) mol^?1) in different mixture types (monospecific (M): a Norway spruce seedling surrounded by six spruce seedlings, group-admixture (G): a spruce seedling surrounded by three spruce and three European beech seedlings, single-admixture (S): a spruce seedling surrounded by six beech seedlings). After seven years of treatments, no significant effect from elevated CO₂ was found on the root dry mass (p?=?0.90) and radial growth (p?=?0.98) of Norway spruce. Neither did we find a significant interaction between [CO₂]?×?mixing treatments (p?=?0.56), i.e. there was not a significant effect of CO₂ concentrations [CO₂] in all the admixture types. On the contrary, spruce responses to admixture treatments were significant under CO_2AMB (p?=?0.05), which demonstrated that spruce mainly increased its growth (diameter and root) in M and neighbouring with beech was not favourable for spruce seedlings. In particular, spruce growth diminished when growing beside high proportions/numbers of European beech (S). Here, we also evaluated the association between tree-ring formation and climatic variables (precipitation and air temperature) in different admixture types under elevated and ambient CO₂ (CO_2AMB, 385 μmol (CO₂) mol^?1). Overall, our result suggests that spruce responses to climate factors can be affected by tree species mixing and CO₂ concentrations, i.e. the interaction between climatic variables?×?admixture types?×?[CO₂] could alter the response of spruce to climatic variables.

     

Ionic composition and osmolality of paddlefish (Polyodon spathula, Acipenseriformes) seminal fluid

Changes in ionic composition as Na⁺,K⁺, Ca²⁺ and Mg²⁺, osmolality inseminal fluid, percentage of motile spermatozoaand velocity were investigated in response toCPP and different dosage of LHRHa. The lowestvelocity of sperm was observed after use CPPtreatment. The velocity of spermatozoa,significant main effect of the treatment(P < 0.0001) and the time of sperm collection(P < 0.0104) were evaluated. The osmolality ofseminal fluid was different betweenexperimental groups of LHRHa (48.0–62.7mOsmol.kg^–1) and CPP (33.0–46.3mOsmol.kg^–1) treatments. The osmolalitywas significantly higher on the first day andone-half, then declined on day three, rangingfrom 33.0 to 62.7 mOsmol.kg^–1. Analysisof variance showed significant main effects ofthe treatment (P < 0.0001) and the time ofsperm collection (P < 0.0002) on the osmolalityof seminal fluid. The level of Na⁺ andK⁺ ion was different between experimentalgroups of LHRHa and CPP treatment. The highestconcentration of 11.11 mmol.l^–1 wasobserved at Na⁺ ion. Then theconcentrations declined on the level 1.56, 0.52and 0.36 mmol.l^–1 for K⁺, Ca²⁺and Mg²⁺ ions, respectively. There werehighly positive correlations between osmolalityof seminal fluid and dosage of LHRHa treatment(r = 0.84), velocity of spermatozoa andosmolality of seminal fluid (r = 0.57) andosmolality of seminal fluid and Na⁺concentration at seminal fluid (r = 0.70).Injection with LHRHa increased quality of spermas velocity of sperm, level of Na⁺,K⁺ and osmolality at seminal fluidcompared to CPP treatments.     

The effects of different types of management,functions, and characteristics of stands in Polish forests on the amount of coarse woody debris

In the modern forestry paradigm, many factors influence the amount of coarse woody debris (CWD). The present paper analyzes the effects of both local (national) programs (special functions of forests) and European programs (Natura 2000 sites), as well as the individual characteristics of forest stands. The study was conducted on 2,752 sampling plots distributed over an area of about 17,500 ha and located in lowland stands having a species composition typical of large areas in central Europe. Natura 2000 areas contained significantly more CWD (8.4 m³/ha) than areas not covered by the program (4.8 m³/ha). However, this is due to the fact that Natura 2000 sites involve well-preserved forest areas, such as nature reserves (26.6 m³/ha). In the managed forests that have been covered by the Natura 2000 program over the past several years, the volume of CWD has not increased. Forests with ecological and social functions differed slightly in the amount of CWD. More CWD occurred in protected animal areas (8.7 m³/ha) than in stands damaged by industry (3.9 m³/ha). Intermediate CWD levels were found in water-protection forests and in forests located around cities and military facilities. In managed forests, the lowest CWD volume was observed in middle-aged stands. The species composition of the stand had little effect on the volume of CWD. Only stands with a predominance of ash and alder had higher CWD levels (13.5 m³/ha). More CWD was found in stands whose species composition did not represent the potential site quality (6.4 m³/ha) than in habitats with the optimum species composition (3.8 m³/ha). CWD volume should be systematically increased taking into consideration local natural conditions. Such efforts should be focused on particularly valuable regions, and especially on Natura 2000 sites, where the threshold values reported from other European forests should be reached. Leaving some trees to die naturally and retaining reasonable amounts of such trees ought to be incorporated into CWD management practice in Poland.     

Scots Pine (Pinus sylvestris L.) Growth Suppression and Adverse Effects on Human Health Due to Air Pollution in the Upper Silesian Industrial District (USID), Southern Poland

Air pollution emissions were not continually monitored in the Upper Silesian Industrial District (USID), southern Poland, and data is only available for the last 20?years. Long-lasting and severe tree ring reductions in pines growing 5-20 km north of the USID area recorded particularly high levels of air pollution emissions in the period 1950-1990. Especially high amounts of reductions and many missing rings were found in the period 1964-1981. At the same time, pines growing 60?km west of the USID do not record deep ring reductions; this proves that the phenomenon is of a regional nature. Increases in infant mortality and lung, bronchial, and tracheal cancer morbidity rates among males were also recorded in the USID during periods of high air pollution. Infant mortality rates increased several years after the tree ring reductions. Therefore, it may be possible to use tree ring reductions as an early indicator of the occurrence of adverse effects on human health.     

Ethnobotanical knowledge and agrobiodiversity in subsistence farming: case study of home gardens in Phong My commune, central Vietnam

Ethnobotanical survey was conducted in 101 randomly selected home gardens of Phong My commune, central Vietnam, situated in the buffer-zone of Natural Reserve. Data were collected through semi-structured questionnaires and direct observation. Sixty-seven species belonging to 35 families were identified to be used for various purposes. For each species the botanical and vernacular names, plant parts used and main purposes of use are given. The major use categories reported for plant species were food (86%), medicine (32%) and firewood (32%), however, seventy-seven percent of all inventoried plants have multiple uses. The species Artocarpus heterophyllus Lam., Citrus grandis Osbeck, Citrus reticulata Blanco, Ipomoea batatas (L.) Lam. and Musa spp. were identified as the main sources of plant foods consumed within the households. Statistical analyses indicated by Shannon-Wiener and Margalef indices have shown that local species diversity and richness is affected by home garden size. On the contrary, no relationship between diversity and home garden age was found. On the basis of a cluster analysis of plant species diversity, five home garden types were differentiated. In comparison to previously reported studies on tropical home gardens, the diversity in Phong My is lower, probably due to market-oriented strategy. Nevertheless, based on the results achieved, we can conclude that useful plants cultivated in local home gardens provide valuable foods complementing daily diet and subsequently contributing to socioeconomic status of the households.     

Newly identified host range of European mountain ash ringspot‐associated virus (EMARaV) and its distribution in the Czech Republic

It has been previously shown that European mountain ash ringspot‐associated virus (EMARaV), a multipartite, negative‐strand RNA plant virus, is pathogenic for European mountain ash (Sorbus aucuparia L.). However, its transmissibility to and replication in other host species remains poorly understood. In this work, we performed experimental inoculations of seventeen virus‐tested species and interspecies hybrids of subfamily Maloideae (family: Rosaceae) with buds from EMARaV‐infected S. aucuparia trees. Surprisingly, a majority of the graft‐inoculated tree species, for instance Sorbaronia, Aronia and Amelanchier, developed symptoms typical for EMARaV infection. These symptoms included chlorotic spots of various size and shape and mottling on leaves but not on flowers and fruits. The propagation of EMARaV in graft‐inoculated tree species was subsequently confirmed by molecular detection tools. Based on these results, we carried out a systematic survey throughout the Czech Republic and identified the presence of EMARaV in wild growing trees in all explored regions, with massive disease infestation in several locations. More importantly, EMARaV infection was newly detected in S. aucuparia subsp. moravica, S. aucuparia × Crataegus sanguinea cv. Granatnaja, (Sorbus aria × Aronia arbutifolia) × S. aucuparia cv. Burka, cv. Finskaja (a Sorbus hybrid of unknown origin) and Aronia melanocarpa; all these EMARaV‐infested trees were grown in production orchards. The sequencing of the nucleocapsid (NC)‐coding gene from several of these EMARaV isolates provided insight into the population structure and phylogenetic relationships of the EMARaV variants occurring in the Czech Republic. Together, our biological and molecular findings extend our knowledge on the host range of EMARaV and provide the first evidence of its natural occurrence in hosts dissimilar to S. aucuparia.